Manipulating the Mouse Embryo: A Laboratory Manual

Chapter 1. Developmental Genetics and Embryology of the Mouse: Past, Present, and Future Chapter 2. Summary of Mouse Development Chapter 3. Production of Transgenic and Chimeric Mice: General Issues Chapter 4. Recovery and In Vitro Culture of Pre-implantation-Stage Embryos Chapter 5. Isolation, Culture, and Manipulation of Post-implantation Embryos Chapter 6. Surgical Procedures Chapter 7. Production of Transgenic Mice Chapter 8. Isolation, Culture, and Manipulation of Blastocyst-derived Stem Cells Chapter 9. Vector Designs for Embryonic Stem Cell-based Transgenesis and Genome Alterations Chapter 10. Introduction of Foreign DNA into Embryonic Stem Cells Chapter 11. Production of Chimeras Chapter 12. Detection and Analysis of Mouse Genome Alterations and Specific Sequences Chapter 13. Parthenogenesis, Pronuclear Transfer, and Mouse Cloning Chapter 14. Assisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial Insemination, and Intracytoplasmic Sperm Injection Chapter 15. Cryopreservation, Rederivation, and Transport of Mice Chapter 16. Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems Chapter 17. Setting up and Equipping a Micromanipulation Lab Appendices