Direct Visualization of Lipid Phase Segregation in Single Lipid Bilayers with Coherent Anti‐Stokes Raman Scattering Microscopy

Imaging of giant vesicles: Coherent anti-Stokes Raman scattering (CARS) microscopy is used for imaging of giant vesicles of binary mixtures of lipids. The vibrational selectivity of the CARS microscope allows determination of molecules based on differences in the vibrational modes. The picture shows Raman and CARS spectra as well as CARS images of a deuterated giant unilamellar vesicle.