Expression of miRNA-181 in Hepatocellular Carcinoma

Objective: To investigate the expression of miRNA-181 in normal human hepatocytes(L02),hepatpcellular carcinoma cell lines(SMMC722 and Hep3B),normal liver tissues and hepatocellular carcinoma tissues for explore the role of miRNA-181 in hepatocellular carcinoma(HCC).Methods: MiRNA181,including miRNA-181a/181b/181c/181d in normal human hepatocytes(L02),hepatpcellular carcinoma cell line(SMMC722 and Hep3B),normal liver tissues and hepatocellular carcinoma tissues were tested by florescent real-time quantitative PCR.Results: Compared to in the normal hepatocytes(L02),the level of miRNA-19b was markedly increased in SMMC722 and Hep3B(P0.05 or P0.001).Compared to in normal liver tissues,the level of miRNA-19b was markedly increased in hepatocellular carcinoma tissues(P0.05).Conclusions: The expression of miRNA-19b was different in various hepatpcellular carcinoma cell lines,and increased than normal hepatocytes.The expression of miRNA-19b in hepatocellular carcinoma tissues was increased than in normal liver tissues.It probably involved in the progress of HCC and was the marker of diagnosis and prognosis of hepatocellular carcinoma.