Study on Expression Conditions of EGCG-O-Methyltransferase in Recombinant Escherichia coli Bacteria

EGCG3Me could be produced from EGCG catalyzed by EGCG-O-Methyltransferase.Taken the yield of EGCG3Me as main index,the present study focused on the producing conditions of EGCG-O-Methyltransferase induced by IPTG in recombinant E.coli bacteria.Results showed that the optimum producing conditions were as follows: the concentration of IPTG was 0.05 mmol/L,the induction time was 20 h,the initial pH of medium was 7.0 and the induction temperature was 20℃.