Determination of betamethasone contents in Betamethasone-urea cream by HPLC

OBJECTIVE To estabtish an HPLC method to control the quality of Betamethasone-urea cream, HPLC method was established to determine betamethasone. METHODS The chromatographic system consisted of VP-ODS column and methanol-water(65∶35) as the mobile phase with a detection wavelength at 240 nm. RESULTS Betamethasone could islated from other material by HPLC. The linear range of betamethasone was 2.6-100.8 μg·ml -1 (r=0.9999). The recovery rate was 99.46% with RSD of 0.66%(n=9). CONCLUSION This method is exclusive , accurate and simple for contents determination of Betamethasone-urea cream.