[Establishment and evaluation of an animal model of female pelvic nerve injury].

Objective: To build a stable animal model simulating pelvic nerve injury in female pelvic floor dysfunction. Methods: A total of 55 10-week-old female SD rats weighing (220±15) g were randomly divided into 3 groups: 5 for normal group, 25 for sham operation group (SO), 25 for bilateral pudendal nerve block group (BPNB). Samples of rat anterior vaginal wall were obtained in 3 days, 1 week, 1 month and 3 months after the operation. The number of nerve fibers was counted per high power field under microscope, with UCHL immunohistochemical staining of nerve fibers. RNA was extracted and the expression of RNA related to nerve tissue was tested. Results: The numbers of nerve fibers had no significant difference between the normal group and the sham operation group. The numbers of nerve fibers in anterior vaginal wall of rats in BPNB group, was obviously decreased in 3 days after the operation, reached a minimum value at 1 weeks, and lasting till 3 months. QRT-PCR indicated that the expression of UCHL mRNA in the BPNB group was significantly decreased after 1 week, 1 month and 3 months, while the expression of Nestin was significantly decreased 1 month and 3 months after the operation. Conclusions: Bilateral pudendal nerve block could be used to make rat models of anterior vaginal nerve injury for further exploratory research on pelvic nerve injury theory of pelvic floor dysfunction.目的： 探索建立稳定的盆底神经损伤动物模型，用于女性盆底功能障碍性疾病神经损伤机制及治疗方法的研究。 方法： 选取55只10周龄未生育SD大鼠，大鼠体质量（220±15）g，雌性。随机数字表法将大鼠分为3组：正常组5只、假手术组（SO）25只和动物模型组（BPNB）25只。正常组大鼠于实验开始时处死并进行阴道前壁的取材，SO组和BPNB组分别于术后3 d、1周、1和3个月处死并进行阴道前壁取材进行组织学、分子检测分析。每组每时间点处死5只。 结果： BPNB组大鼠阴道前壁神经纤维数量较SO组自术后第3天明显降低，至术后1周降至最低值，并可维持至术后3个月；BPNB组大鼠前壁泛素羧基端水解酶（UCHL）mRNA表达水平术后1周、1、3个月均明显低于SO组，巢蛋白（Nestin）mRNA表达水平术后1、3个月明显低于SO组。 结论： 双侧阴部神经阻断法制备的盆底神经损伤大鼠模型，可作为盆底障碍性疾病盆底神经损伤较为稳定、实用的动物模型。.