基因组编辑
清脆的
Cas9
生物
基因组
大肠杆菌
遗传学
基因
计算生物学
基因组工程
化脓性链球菌
质粒
细菌基因组大小
肠杆菌科
细菌
金黄色葡萄球菌
作者
Yu Jiang,Biao Chen,Chunlan Duan,Bingbing Sun,Junjie Yang,Sheng Yang
摘要
An efficient genome-scale editing tool is required for construction of industrially useful microbes. We describe a targeted, continual multigene editing strategy that was applied to the Escherichia coli genome by using the Streptococcus pyogenes type II CRISPR-Cas9 system to realize a variety of precise genome modifications, including gene deletion and insertion, with a highest efficiency of 100%, which was able to achieve simultaneous multigene editing of up to three targets. The system also demonstrated successful targeted chromosomal deletions in Tatumella citrea, another species of the Enterobacteriaceae, with highest efficiency of 100%.
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