Effect of X-ray radiation on the expression of TNF-α and NF-κB in the RAW264.7 macrophage cell

OBJECTIVE To investigate the regularity of the X-ray irradiation induced murine macrophage secretion of TNF-α and NF-кB.METHODS Murine macrophage RAW264.7 were in single fraction irradiation of 8Gy X-ray.Then cell supernatant was collected on different time by 8Gy X-ray.The secretion of TNF-α and the expression of NF-κB were assayed by ELISA and Western blot,respectively.RESULTS TNF-α level was increased by X-ray irradiation,which was reached a peak secretion after irradiation on 34h.NF-κB(p65)distribution was increased,which was reached a maxlmum after irradiation on 4h.With the time prolongation,its nuclear translocation was obviously increased.CONCLUSION The expression of TNF-α and NF-κB were induced by 8Gy X-ray in murine macrophage RAW264.7.