Transformation of protopanaxadiol saponin to ginsenoside-Rg_3 by Phycicoccus sp.BXN5-13

The ginsenosidase was produced by Phycicoccus sp.BXN5-13 for transformation of Rb1 and other protopanaxadiol saponin to the ginsenoside Rd and Rg3.The ginsenosidase was an intracellular enzyme that produced maximally when cultured in the medium containing 10 g/L triptone,5 g/L yeast extract and 10 g/L NaCl and ginsenoside at 35 ℃ for 72 h.The optimal condition for enzymatic hydrolysis of ginsenoside was as pH 6.0 at 45 ℃ for 24 h.The transformation rate of ginsenoside-Rg3 was 44%.