Changes of expression of matrix metalloproteinase-9 and S100B of brain tissue in intracerebral hemorrhage rats

Objective To study the changes of expression of matrix metalloproteinase-9 (MMP-9) and S100B of brain tissue in intracerebral hemorrhage(ICH) rats.Methods 64 SD rats were randomly divided into ICH 3 d,7 d,14 d and 21 d groups,and there were 16 rats in each group.ICH models were made by autoblood injection method.The concentration of MMP-9 and S100B including protein and mRNA were detected by immunohistochemical method and in situ hybridization method.Results MMP-9 expression was significantly higher in the ICH 3 d group than that in the other three groups(all P0.05).There was no significant difference among ICH 7 d,14 d and 21 d groups.S100B expression was a little in ICH 3 d group,and there were statistical differences between ICH 7 d group and other three groups (all P0.05).The expression of S100B were obviously decreased in ICH 14 d and 21 d groups.Conclusions MMPP-9 and S100B are both participate in the pathophysiological processes of brain tissue injury after ICH,MMP-9 is an early stage of ICH and S100B is a intermediate stage of ICH damage criterion of brain tissue.