Effects of house dust mites with diverse concentrations on the expression co-stimulatory molecules of Der p2-modified dendritic cell

Objective To investigate the effects of house dust mites (HDM) with diverse concentrations on the expressions of co-stimulatory molecules of HDM major antigen gene Der p2 modified dendritic cell(DC). Methods Der p2 gene modified DC(Der p2-DC) and control DC(non-transfected DC)were treated 72 h by H DM with diverse concentrations respectively. The expressions of CD80,CD86 and MHC Ⅱ ,the surface co-stimulatory molecules on Der p2-DC and control DC, were detected by FACS.Results FACS analysis showed that positive expression percentages of CD80,CD86 and MHC Ⅱ on Der p2-DC treated with HDM had no significant change,compared with Der p2-DC treated without HDM.After treated with HDM, positive expression percentages of CD80, CD86 and MHC Ⅱ on control DC increased significantly. Conclusions Specific allergen pulsing could not increase the expressions of costimulatory molecules of allergen gene modified DC. Key words: Dendritic cell; Co-stimulatory molecule; Allergen; Immune tolerance