内科学
内分泌学
放射免疫分析
胆固醇侧链裂解酶
邻苯二甲酸盐
颗粒细胞
卵巢
细胞生长
类固醇激素
活力测定
生物
化学
激素
体外
细胞色素P450
新陈代谢
医学
生物化学
有机化学
作者
Na Li,Te Liu,Kun Guo,Jian Zhu,Ge Yu,Shuyue Wang,Lin Ye
摘要
This study aimed to examine the proliferation of and secretion by rat ovarian granulosa cells (GCs) treated with mono‐(2‐ethylhexyl) phthalate (MEHP). Ovarian GCs were incubated with MEHP at concentration of 0, 25, 50, 100, and 200 µM for 24 hr. Cell viability was determined using the MTT Cell Proliferation Assay. Progesterone and estradiol production was evaluated by radioimmunoassay (RIA) and the expression of FSHR, PR, and ER was measured by immunocytochemistry. StAR, P450scc, 3β‐HSD, 17β‐HSD, and P 450 arom mRNA levels were determined by RT‐PCR. MEHP markedly attenuated proliferation of GCs, increased expression of sex hormone receptors and key enzymes in progesterone production, and stimulated steroid hormone secretion. The result of these analyses demonstrates that MEHP exposure of GCs may have effects on rat ovarian functions.
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