Study on Enzymatic Synthesis of γ-Cyclodextrin with β-Cyclodextrin

Gamma cyclodextrin was produced synthesized from beta cyclodextrin in the presence of glycyrrhizin, using Cyclodextrin glucanotransferase (EC 2.4.1.19) from Alkalophilic Bacillus . The results showed that more than 32.7% of beta cyclodextrin substrate was converted to the corresponding gamma cyclodextrin under the following conditions, viz. 200 units enzyme/g of β-CD, 10mmol/L of β-CD concentration, 3% of glycyrrhizin concentration, 27 h of reaction time, 65°C of temperature and a reaction pH of 7.0.