Time-dependent impact of a high-fat diet on the intestinal barrier of male mice
食品科学
生物
化学
内科学
内分泌学
医学
作者
Carolline Santos Miranda,Daiana Araujo Santana-Oliveira,Isabela Lopes Vasques-Monteiro,Nathan Soares Dantas-Miranda,Jade Sancha de Oliveira Glauser,Flávia Maria Silva-Veiga,Vanessa Souza‐Mello
出处
期刊:World journal of methodology [Baishideng Publishing Group Co (World Journal of Methodology)] 日期:2024-03-20卷期号:14 (1)
标识
DOI:10.5662/wjm.v14.i1.89723
摘要
BACKGROUND Excessive saturated fat intake compromises the integrity of the intestinal mucosa, leading to low-grade inflammation, impaired mucosal integrity, and increased intestinal permeability, resulting in the migration of lipopolysaccharide (LPS) to other tissues. AIM To evaluate the chronic effects (at 10 and 16 wk) of a high-fat diet (HFD) (with 50% energy as fat) on the phylogenetic gut microbiota distribution and intestinal barrier structure and protection in C57BL/6 mice. METHODS Forty adult male mice were divided into four nutritional groups, where the letters refer to the type of diet (control and HFD or HF) and the numbers refer to the period (in weeks) of diet administration: Control diet for 10 wk, HFD for 10 wk, control diet for 16 wk, and HFD for 16 wk. After sacrifice, biochemical, molecular, and stereological analyses were performed. RESULTS The HF groups were overweight, had gut dysbiosis, had a progressive decrease in occludin immunostaining, and had increased LPS concentrations. Dietary progression reduced the number of goblet cells per large intestine area and Mucin2 expression in the HF16 group, consistent with a completely disarranged intestinal ultrastructure after 16 wk of HFD intake. CONCLUSION Chronic HFD intake causes overweight, gut dysbiosis, and morphological and functional alterations of the intestinal barrier after 10 or 16 wk. Time-dependent reductions in goblet cell numerical density and mucus production have emerged as targets for countering obesity-driven intestinal damage.