高尔基体
氧化应激
荧光
化学
氧化磷酸化
生物物理学
细胞生物学
生物化学
生物
物理
内质网
光学
作者
Hui Wang,Zixu He,Yuyun Yang,Jiao Zhang,Zhang Wei,Wen Zhang,Ping Li,Bo Tang
出处
期刊:Chemical Science
[The Royal Society of Chemistry]
日期:2019-01-01
卷期号:10 (47): 10876-10880
被引量:78
摘要
Golgi oxidative stress is significantly associated with the occurrence and progression of hypertension. Notably, the concentration of hydrogen peroxide (H2O2) is directly proportional to the degree of Golgi oxidative stress. Therefore, based on a novel Golgi-targeting phenylsulfonamide group, we developed a two-photon (TP) fluorescent probe, Np-Golgi, for in situ H2O2 ratiometric imaging in living systems. The phenylsulfonamide moiety effectively assists Np-Golgi in the precise location of Golgi apparatus. In addition, the raw material of phenylsulfonamide is easily available, and chemical modification is easily implemented. By application of Np-Golgi, we explored the generation of H2O2 during Golgi oxidative stress, and also successfully revealed increases on the levels of Golgi H2O2 in the kidneys of mice with hypertension. This work provides an ideal tool to monitor Golgi oxidative stress for the first time and novel drug targets for the future treatment of hypertension.
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