The experimental research and theoretical modeling of the erythrocyte refractive index have been carried out. It has been proposed the model of the erythrocyte refractive index determination by means of two wavelengths. It has been shown that the accuracy of the erythroctye refractive index determination increases when the wavelengths decreases. The obtained data of the erythrocyte refractive index on 0,63 and 0,53 micron wavelengths are in a good agreement with a dispersion dependence of the erythrocyte refractive index. The value of the obtained refractive index by means of two wavelengths method are in a good agreement with the refractive index of the erythrocyte on 0,63 and 0,53 micron wavelengths.