Objective To analyze the distribution of pathogenic bacteria isolated from our hospital and the antibiotic resistance of the main pathogenic bacteria,to provide a basis for clinical rational drug use and control the hospital infection.Methods 613 strains of pathogenic bacteria separated from patients in our hospital during June to December of 2012 were identifited by Hengxing bacteria analysis and detected the antibiotic resistance by Kirby-Bauer(K-B) agar tapediffusion.Results We gather 613 strains in last year of 2012,there were 417 strains Gram-negative bacteria(68%),128 strains Gram-positive bacteria(20.8%),and 68 strains fungi(13.2%),obviously the antibiotic resistance of pathogenic bacteria was higer than first half year.Conclusion It is necessary to reasonably use antibiotics,strengthen the awareness of bacteria culture and strictly comply the standards of diagnosis and antibiotic use so as to prevent the antibiotic resistanc of pathogenic bacteria.Gram-negative bacteria was the main pathogenic bacteria,clinician should ware the infection of Gram-negative bacteria.