Objective: To observe the effect of three kinds of surgery: suprahigh ligation,high ligation and transinguinal ligation of internal spermatic vein.Methods: 136 patients with degree Ⅱ varicocele were enrolled and divided into 3 groups: suprahigh ligation group(42 cases),high ligation group(46 cases),transinguinal ligation group(48 cases).All the densities,the movements and the structures of the sperms were follow-up before and after operation.Results: Suprahigh ligation group was better than that of high ligation group in densities and structures of the sperms,suprahigh ligation group and high ligation group surpasses that of transinguinal ligation group in densities,movements and the structures of the sperms.Conclusion: Suprahigh ligation of internal spermatic vein is more conducive to the resumption of semen quality.