IN VITRO STUDY ON THE EFFECT OF PROPOLIS ON THE GROWTH AND ADHERENCE OF S.MUTANS

Objective To study the effect of propolis on growth and adherence of S.mutans.Methods (1)The experiment of inhibiting the growth of cariogenic bacteria was performed.The minimum inhibitory concentration of propolis extracts to inhibit S.mutans and S.sobrinus was assayed by means of using stairless-steel cylinders with proportional diluted watersoluble propolis and ethamolic extract of propolis.(2) The experiment of inhibiting the adherence of cariogenic bacteria S.mutans were culture continuously in different MS liquid culture medium which contained various concentration of watersoluble propolis,ethamolic extract of propolis,chlorhexdine and ethanol.After 8 days,the weight of the cells of S.mutans which was adhered on the capillary surface was measured and compared.Results The minimum inhibitary concentrations of propolis extracts to S.mutans and S.sobrinus were 0.031625%,0.25% watersoluble propolis,0.078% ethamolic extract of propolis respectively.Each group of Propolis extract and chlorhexdine significantly inhibited the adherence of S.mutans (P0.05).The effect of the groups of 0.5% watersoluble propolis,ethamolic extract of propolis and chlorhexdine were stronger whereas there was no significant differences among three groups(P0.05).Conclusion Both the watersoluble propolis and ethamolic extract of propolis have significant effects on inhibiting the growth and adherence of S.mutans.