Example dataset for “4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination”

These are representative test data for the paper "4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination".Cell03_561_000_000.dcimg: A subset of the raw data for the microtubules in Fig. 2, 18,000 frames.align_561_FMTtransform_20250125.mat: Beads data for multi-channel registration.bead_561_Astfit_20250125.mat: Beads data for astigmatism calibration.bead_561_dphi_cali_20250125.mat: Beads data for phase delay calibration.Note: 4Pi-SIMFLUX reconstruction code is available at: https://github.com/zhanglab-srm/4Pi-SIMFLUX.