Study on the Integration of T-DNA into Lilium longiflorum

[Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. [Result] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6-0.8,the addition of 250 mg/L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg/L. Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR,preliminarily proving that T-DNA gene had integrated into the genome of lily. [Conclusion] The study may lay a foundation for breeding excellent lily varieties through T-DNA integration technique.