Comparison of Five PCR Template Preparation Methods for Fast Identification of Actinobacteria

The 23S rRNA gene of Actinobacteria is always considered as a suitable location for fast screening Actinobacteria from bacteria because it is conserved and variable.It is critical to get high quality samples before PCR amplification.Therefore five genomic DNA extraction methods were compared with each other.The aim of this study is to search one new method, which could be used as accurately,fast,easily and economically screening Actinobacteria,providing a platform for exploration and utilization Actinobacteria resources under normal and extreme environments.