Study on the Isolation and Identification of Brucella in Dairy Cows

To investigate the prevalence of Brucella in Xinjiang,serum samples collected from 3 dairy farms were tested by serum agglutination test(SAT) for the detection of infected antibodies to Brucellosis.Two isolated strains which identified by VirB8-PCR were got from milk samples of positive cow by using serum agglutination test(SAT) of a cattle farm in Urumqi region.The results indicated that the isolated strains were Brucella.In addition to the AMOS-PCR assays,isolated strains of Brucella specifically were identified for B.abortus.Furthermore,it differentiated B.abortus biovars 3b,5,6 and 9.