粒体自噬
自噬
细胞生物学
生物
细胞器
故障排除
体内
线粒体
机制(生物学)
计算生物学
计算机科学
遗传学
细胞凋亡
物理
量子力学
操作系统
作者
Thomas G. McWilliams,Ian G. Ganley
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 621-642
被引量:15
标识
DOI:10.1007/978-1-4939-8873-0_41
摘要
Autophagy evolved as a mechanism to sustain cellular homeostasis during instances of nutrient deprivation. Mounting evidence has also clarified that under basal and stress conditions, selective autophagy pathways can target the destruction of specific organelles. Mitochondrial autophagy, or mitophagy, has emerged as a key quality control (QC) mechanism to sustain the integrity of eukaryotic mitochondrial networks. We recently reported the development of mito-QC, a novel reporter mouse model that enables the high-resolution study of mammalian mitophagy with precision, in fixed and live preparations. This model holds significant potential to transform our understanding of mammalian mitophagy pathways in vivo, in a variety of physiological contexts. We outline a detailed protocol for use of our recently described mito-QC mouse model, including tips and troubleshooting advice for those interested in monitoring mitophagy in vitro and in vivo.
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