Determination of Lignin and Cellulose in Acid-Detergent Fiber with Permanganate

Abstract A newly developed indirect method for lignin, utilizing permanganate, permits the determination of cellulose and insoluble ash in the same sample. The new permanganate lignin method is intended as an alternative procedure to the 72% sulfuric acid method over which it offers definite advantages as well as certain disadvantages. Choice of methods will depend upon the materials analyzed and the purpose for which the values are to be used.