反向遗传学
生物
基因组
病毒学
遗传学
病毒
病毒复制
质粒
突变体
分子遗传学
基因
作者
Johnasha D. Stuart,Matthew B. Phillips,Karl W. Boehme
出处
期刊:Springer eBooks
[Springer Nature]
日期:2017-01-01
卷期号:: 1-10
被引量:3
标识
DOI:10.1007/978-1-4939-6964-7_1
摘要
Reverse genetics allows introduction of specific alterations into a viral genome. Studies performed with mutant viruses generated using reverse genetics approaches have contributed immeasurably to our understanding of viral replication and pathogenesis, and also have led to development of novel vaccines and virus-based vectors. Here, we describe the reverse genetics system that allows for production and recovery of mammalian orthoreovirus, a double-stranded (ds) RNA virus, from plasmids that encode the viral genome.
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