Study on effect and mechanism of Anchang Yuyang Decoction with Jiawei Shengji Powder on ulcerative colitis in rats.

Objective:To study effect and mechanism of Anchang Yuyang Decoction with Jiawei Shengji Powder (ACYYDJWSJP) on ulcerative colitis (UC) in rats. Methods: Ninety SD rats were divided randomly into six groups: normal control group,pathologic model control group,ACYYD group, JWSJP group, ACYYD JWSJP group And Solfasalazine (SASP) treatment group. The model of ulcerative colitis were established by given 2,4,6-TNBS. After medication for 3 weeks, the parameters including colonic gross morphology, colon tissue injury scores, MPO activity, the content of IL-1β, IL-8, TNF-α and IL-10 were observed. Results: Inflammation relieving of colonic mucosa and ulcer healing were found in all treatment group rats at different degree. Colonic gross morphology, colon tissue injury scores,MPO activity and the level of IL-1β,IL-8, TNF-α decreased, while levels of IL-10 increased after therapy (P0.05 or P0.01), and there was significant difference among groups,especially between ACYYD JWSJP treatment group and pathologic model control group. Conclusion: Research showed that ACYYD JWSJP had made a good effect. The mechanism might be down-regulating the expressions of pro-inflammatory cytokines (IL-1β,IL-8,TNF-α) and upregulating the expressions of anti-inflammatory cytokine (IL-10), which can then adjust the immune system, inhibit the inflammation reaction and promote ulcer healing.