开放式参考框架
核糖体分析
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核糖体
基因座(遗传学)
翻译(生物学)
遗传学
计算机科学
计算生物学
生物
基因
信使核糖核酸
核糖核酸
肽序列
作者
Dermot Harnett,Eelco Meerdink,Lorenzo Calviello,Dominique Sydow,Uwe Ohler
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 331-346
被引量:4
标识
DOI:10.1007/978-1-0716-1150-0_16
摘要
Ribosome profiling, or Ribo-seq, provides precise information about the position of actively translating ribosomes. It can be used to identify open reading frames (ORFs) that are translated in a given sample. The RiboTaper pipeline, and the ORFquant R package, leverages the periodic distribution of such ribosomes along the ORF to perform a statistically robust test for translation which is insensitive to aperiodic noise and provides a statistically robust measure of translation. In addition to accounting for complex loci with overlapping ORFs, ORFquant is also able to use Ribo-seq as a tool for distinguishing actively translated transcripts from non-translated ones, within a given gene locus.
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