The antifungal metabolites isolated from maize endophytic fungus Fusarium sp. induced by OSMAC strategy

Six new sesquiterpenes, fusarchlamols A-F (1, 2, 4–7); one new natural product of sesquiterpenoid, methyltricinonoate (3); and ten known compounds were found from Fusarium sp. cultured in two different media by the one strain many compounds strategy. The compounds (1, 2, and 4–11) were isolated from Fusarium sp. in PDB medium, and compounds (3–5, 8, and 10–17) were discovered from Fusarium sp. in coffee medium. Additionally, the configuration of 8 was first reported in the research by Mosher's method. The structures were established by 1D, 2D NMR, mass spectrometry, calculated ECD spectra, and Mosher's method. Compounds 1, 2, 6/7, 12, and 16 indicated significant antifungal activities against the phytopathogen Alternaria alternata isolated from Coffea arabica with MICs of 1 μg/mL. The investigation on the anti-phytopathogen activity of metabolites can provide lead compounds for agrochemicals.