胰岛素
激素
内皮干细胞
胰岛素受体
受体
内科学
内分泌学
细胞生物学
化学
生物
医学
生物化学
体外
胰岛素抵抗
作者
George L. King,Sandra Marchese Johnson
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1985-03-29
卷期号:227 (4694): 1583-1586
被引量:441
标识
DOI:10.1126/science.3883490
摘要
Hormones such as insulin are transported from the interior to the exterior of blood vessels. Whether endothelial cells, which line the inner walls of blood vessels have a role in this transport of hormones is not clear, but it is known that endothelial cells can internalize and release insulin rapidly with little degradation. The transport of iodine-125-labeled insulin was measured directly through the use of dual chambers separated by a horizontal monolayer of cultured bovine aortic endothelial cells. In this setting, endothelial cells took up and released the labeled insulin, thereby transporting it across the cells. The transport of insulin across the endothelial cells was temperature sensitive and was inhibited by unlabeled insulin and by antibody to insulin receptor in proportion to the ability of these substances to inhibit insulin binding to its receptor. More than 80 percent of the transported insulin was intact. These data suggest that insulin is rapidly transported across endothelial cells by a receptor-mediated process.
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