微塑料
消化(炼金术)
化学
环境化学
厌氧消化
制浆造纸工业
环境科学
色谱法
甲烷
有机化学
工程类
作者
Lisa W. von Friesen,Maria Granberg,Martin Hassellöv,Geir Wing Gabrielsen,Kerstin Magnusson
标识
DOI:10.1016/j.marpolbul.2019.03.016
摘要
Standardized methods for the digestion of biota for microplastic analysis are currently lacking. Chemical methods can be effective, but can also cause damage to some polymers. Enzymatic methods are known to be gentler, but often laborious, expensive and time consuming. A novel tissue digestion method with pancreatic enzymes and a pH buffer (Tris) is here presented in a comparison to a commonly applied digestion protocol with potassium hydroxide. The novel protocol demonstrates a highly efficient removal of bivalve tissue (97.7 ± 0.2% dry weight loss) already over-night. Furthermore, it induces no impairment in terms of ability to correctly identify four pre-weathered plastic polymers and six textile fiber polymers by Fourier transform infrared spectroscopy after exposure. The high-throughput protocol requires minimal handling, is of low cost and does not pose risk to the performer or the environment. It is therefore suggested as a candidate for a standardized digestion protocol, enabling successful analysis of microplastics ingested by bivalves.
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