纳米探针
发光
菁
材料科学
近红外光谱
自体荧光
纳米颗粒
荧光
光化学
持续发光
荧光寿命成像显微镜
分析化学(期刊)
光电子学
光子上转换
光致发光
纳米技术
红外线的
光学
化学
物理
热释光
色谱法
作者
Cong Cao,Xiaobo Zhou,Xue Meng,Chunmiao Han,Wei Feng,Fuyou Li
标识
DOI:10.1021/acsami.9b02008
摘要
The difficulty of near-infrared (NIR) ratiometric detection imaging lies in the lack of high-efficiency NIR probes and the overlapping interference between two emission peaks. To achieve more accurate detection in living organisms, dual NIR-emissive luminescent nanoprobes were designed under the same excitation at 808 nm. The Er3+ ion-doped nanoparticles were employed as a reference with their fluorescence emission at 1525 nm. Meanwhile, a cyanine dye molecule (Cy925) was combined on the surface of nanoparticles as the ClO– recognition site with its NIR emission at 925 nm. The ratiometric nanoprobe relied on the ratio of aforementioned two separated NIR peaks (I925nm/I1525nm), featuring deeper imaging penetration depth and low autofluorescence. This nanoprobe was verified to be sensitive and highly selective to ClO– through photoluminescence titration. The in vitro detection experiment developed reasonable work curves, guaranteeing that we can detect the change in concentration of ClO– in mice limbs with arthritis through in vivo imaging experiments.
科研通智能强力驱动
Strongly Powered by AbleSci AI