The effect of two methods (sevag method and TCA method) to remove protein from hericium erinaceus polysaccharides was compared. The results showed that the TCA method was more effective to remove protein from hericium erinaceus polysaccharides and the polysaccharides loss was less. The molecular weight of hericium erinaceus polysaccharides in which the proteins were removed with TCA method was decreased slightly, the change was unapparent.