氧化应激
化学
谷胱甘肽
消化(炼金术)
抗氧化剂
天竺葵苷
活性氧
山奈酚
细胞毒性
多酚
吹覆盆子
胃肠道
生物化学
食品科学
体外
类黄酮
酶
色谱法
作者
Wei Chen,Hongming Su,Yang Xu,Tao Bao,Xiaodong Zheng
出处
期刊:Food Chemistry
[Elsevier]
日期:2016-04-01
卷期号:196: 943-952
被引量:106
标识
DOI:10.1016/j.foodchem.2015.10.024
摘要
Raspberry is well known as rich source of antioxidants, such as polyphenols and flavonoids. However, after consumption, the antioxidants are subjected to digestive conditions within the gastrointestinal tract that may result in structural and functional alterations. Our previous study indicated that acrylamide (AA)-induced cytotoxicity was associated with oxidative stress. However, the protective effect of wild raspberry extract produced before and after in vitro gastrointestinal digestion against AA-induced oxidative damage is unclear. In the present study, we found that wild raspberry extract produced after digestion (RD) had a pronounced protective effect against AA-induced cytotoxicity compared with that produced before digestion (RE). Further investigation indicated that RD significantly inhibited AA-induced intracellular reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP) collapse and glutathione (GSH) depletion. Moreover, LC-MS analysis revealed that wild raspberry underwent gastrointestinal digestion significantly increased the contents of esculin, kaempferol hexoside and pelargonidin hexoside.
科研通智能强力驱动
Strongly Powered by AbleSci AI