Cryopreservation of Shoot-Tips of Fruit Trees and Herbaceous Plants

Various organisms, when they are sufficiently desiccated, can then be cooled to the temperature of liquid nitrogen (LN) without injury. Extracellular freezing, too, is considered an effective method of dehydrating living cells. Very hardy woody plants, sufficiently dehydrated by extracellular freezing, survived immersion in LN1-3 or in liquid helium 4 when subsequently rewarmed slowly in air at 0°C.