芳香烃受体
G蛋白偶联胆汁酸受体
化学
生物化学
色氨酸
新陈代谢
碳水化合物代谢
脂质代谢
受体
生物
氨基酸
转录因子
基因
作者
Ziwen He,Jingyan Guo,Huiwen Zhang,Jingquan Yu,Yiqing Zhou,Yajun Wang,Ting Li,Ming Yan,Bo Li,Y. Ann Chen,Suhong Chen,Gui‐Yuan Lv,Jie Shi
标识
DOI:10.1016/j.ijbiomac.2023.126987
摘要
Polysaccharides are known to confer protection against glycolipid metabolism disorders (GMD) by regulating intestinal flora. In this study, a heterogeneous acidic heteropolysaccharide with high molecular weight mainly composed of fructose was isolated from Atractylodes macrocephala Koidz (AMP). Supplementation with AMP was shown to improve diet-induced GMD in a rat model, including decreasing the levels of serum triglycerides, total cholesterol, and glucose, and improving hepatic lipidosis and islet cells morphologies. AMP-treated rats also exhibited modified intestinal flora with enrichments of intestinal Lactobacillus and Rothia species, which was accompanied by increased tryptophan metabolites such as indole-3-propionic acid, indole, tryptamine, and tryptophol. These metabolites promote the expression of intestinal aryl hydrocarbon receptor (AhR) in nuclear fractions. AhR activation increased the expression levels of IL-22 and GLP-1 proteins and mRNA. IL-22 reduced systemic LPS by upregulating the expression of tight junction proteins, antimicrobial peptides, and mucin to ameliorate intestinal barrier function, and activated the hepatic IL-22R/Stat3/Acox1 signaling pathway to improve lipid metabolism. GLP-1 activated the pancreatic GLP-1R/p-CREB signaling pathway to ameliorate β-cell injury and improve insulin resistance. Therefore, the intestinal microbial-tryptophan metabolism-AhR pathway was deduced to be a mechanism by which this polysaccharide improves GMD.
科研通智能强力驱动
Strongly Powered by AbleSci AI