纳米孔
放大器
核酸
核酸定量
数字聚合酶链反应
DNA
计算机科学
纳米技术
材料科学
化学
聚合酶链反应
生物化学
基因
作者
Zifan Tang,Gihoon Choi,Reza Nouri,Weihua Guan
出处
期刊:International Electron Devices Meeting
日期:2019-12-01
标识
DOI:10.1109/iedm19573.2019.8993671
摘要
We demonstrate the feasibility of using the single molecule sensing nanopore as a digital counter to enumerate the amplicons for ultrasensitive electronic nucleic acid analysis. We show that the nanopore digital counting approach could capture the DNA replication dynamics in the LAMP reaction and has the potential to be used in a qualitative test as well as in a quantitative test. By keeping nanopores as simple as possible and by leveraging the amplification assay's high sensitivity, the amplification-coupled nanopore digital counting approach provides a promising optics-free method for highly sensitive and specific nucleic acid testing.
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