点头
点头老鼠
胰岛炎
CD11c公司
树突状细胞
生物
免疫学
骨髓
免疫系统
过继性细胞移植
T细胞
体内
自身免疫
生物技术
表型
基因
生物化学
作者
Xiaoqi Zhou,Muyang Yang,Yibing Lv,Heli Li,Sha Wu,Jie Min,Guanxin Shen,Yong He,Ping Lei
标识
DOI:10.1002/jlb.3ma0921-219rrrr
摘要
Abstract The 78-kDa glucose-regulated protein (GRP78) has extracellular, anti-inflammatory properties that can aid resolving inflammation. It has been established previously that GRP78 induced myeloid CD11c+ cell differentiation into distinct tolerogenic cells. This tolerance induction makes GRP78 a potential therapeutic agent for transplanted allogeneic grafts and autoimmune diseases, such as type 1 diabetes. In this research, it is revealed that rmGRP78-treated NOD mice bone marrow-derived CD11c+ cells (GRP78-DCs) highly expressed B7-H4 but down-regulated CD86 and CD40, and retained a tolerogenic signature even after stimulation by LPS. In the assessment of in vivo therapeutic efficacy after the adoptive transfer of GRP78-DCs into NOD mice, fluorescent imaging analyses revealed that the transfer specifically homed in inflamed pancreases, promoting β-cell survival and alleviating insulitis in NOD mice. The adoptive transfer of GRP78-DCs also helped reduce Th1, Th17, and CTL, suppressing inflammatory cytokine production in vivo. The findings suggest that adoptive GRP78-DC transfer is critical to resolving inflammation in NOD mice and may have relevance in a clinical setting.
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