核酸酶
化学
复式(建筑)
检出限
小RNA
生物传感器
胶体金
线性范围
DNA
核酸
分子生物学
纳米技术
计算生物学
纳米颗粒
色谱法
基因
生物化学
生物
材料科学
作者
Bing Bo,Tian Zhang,Yiting Jiang,Haiyan Cui,Peng Miao
标识
DOI:10.1021/acs.analchem.7b05447
摘要
Abnormal expression of miRNAs always occurs in solid tumors. Thus, it is critical to sensitively and selectively detect such biomarkers for the diagnosis and prognosis of diseases. Here, we report a biosensing scheme for the determination of miRNA with triple signal amplification based on target-triggered cyclic duplex specific nuclease digestion and bridge DNA–gold nanoparticles. Electrochemical signals are recorded to present initial levels of miRNA. This method is ultrasensitive with a wide linear range of 10–17 to 10–11 M. The limit of detection is down to 6.8 aM. Moreover, the overexpression of miR-21 is confirmed in lung cancer patients by the proposed method, which is in good accordance with qRT-PCR results. In addition, the developed biosensor does not need a reverse transcription process or any thermal cycling processes. Its performance satisfies the requirement for convenient, rapid, sensitive, and specific early diagnosis of cancers. Therefore, it may have great potential utility in the near future.
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