防御素
丝氨酸蛋白酶
蛋白酶
抗菌肽
抗菌剂
生物化学
微生物学
蛋白酵素
肽
糜蛋白酶
抑制性突触后电位
化学
生物
酶
胰蛋白酶
神经科学
作者
Xudong Luo,Weihua Zhu,Li Ding,Xiangdong Ye,Huanhuan Gao,Xuejiao Tai,Zheng Wu,Yi Qian,Xiuhang Ruan,Jian Li,Shan Li,Zongyun Chen
摘要
Abstract Fungus defensin is a kind of important natural peptide resource, such as plectasin from the soil fungus Pseudoplectania nigrella with potential application in the antimicrobial peptide lead drug discovery. Here, a fungus defensin named Bldesin with Kv1.3 channel and serine protease inhibitory activities was first explored. By GST‐Bldesin fusion expression and enterokinase cleaving strategy, recombinant Bldesin was obtained successfully. Antimicrobial assays showed that Bldesin had potent activity against Gram‐positive Staphylococcus aureus , but had no effect on Gram‐negative Escherichia coli . Electrophysiological experiments showed that Bldesin had Kv1.3 channel inhibitory activity. Serine protease inhibitory associated experiments showed that Bldesin had unique chymotrypsin protease inhibitory, elastase protease inhibitory, and serine protease‐associated coagulation inhibitory activities. To the best of our knowledge, Bldesin is the first functionally characterized pathogenic fungus defensin with Kv1.3 channel and chymotrypsin inhibitory activities and highlighted novel pharmacological effects of fungus‐derived defensin peptides.
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