合子
微量注射
输卵管
转基因
生物
男科
转基因小鼠
微量注射
质粒
DNA
分子生物学
细胞生物学
遗传学
胚胎
基因
医学
胚胎发生
内分泌学
标识
DOI:10.1002/0471142727.mb2309s68
摘要
This unit describes methods for the production of transgenic mice by injection of DNA into zygotes, including fertilized-egg isolation, zygote injection, and oviduct reimplantation. Methods for the preparation of plasmid and BAC DNA suitable for microinjection are also presented.
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