多重耐药
肺结核
结核分枝杆菌
结核分枝杆菌复合物
医学
病毒学
环境卫生
生物
微生物学
抗药性
病理
作者
Albert J. de Neeling,Elisa Tagliani,C Ködmön,Marieke J. van der Werf,Dick van Soolingen,Daniela María Cirillo,Richard Anthony
出处
期刊:Eurosurveillance
[European Centre for Disease Control and Prevention (ECDC)]
日期:2024-03-21
卷期号:29 (12)
标识
DOI:10.2807/1560-7917.es.2024.29.12.2300583
摘要
Background The EUSeqMyTB project, conducted in 2020, used whole genome sequencing (WGS) for surveillance of drug-resistant Mycobacterium tuberculosis in the European Union/European Economic Area (EU/EEA) and identified 56 internationally clustered multidrug-resistant (MDR) tuberculosis (TB) clones. Aim We aimed to define and establish a rapid and computationally simple screening method to identify probable members of the main cross-border MDR-TB clusters in WGS data to facilitate their identification and track their future spread. Methods We screened 34 of the larger cross-border clusters identified in the EuSeqMyTB pilot study (2017–19) for characteristic single nucleotide polymorphism (SNP) signatures that could identify and define members of each cluster. We also linked this analysis with published clusters identified in previous studies and identified more distant genetic relationships between some of the current clusters. Results A panel of 30 characteristic SNPs is presented that can be used as an initial (routine) screen for members of each cluster. For four of the clusters, no unique defining SNP could be identified; three of these are closely related (within approximately 20 SNPs) to one or more other clusters and likely represent a single established MDR-TB clade composed of multiple recent subclusters derived from the previously described ECDC0002 cluster. Conclusion The identified SNP signatures can be integrated into routine pipelines and contribute to the more effective monitoring, rapid and widespread screening for TB. This SNP panel will also support accurate communication between laboratories about previously identified internationally transmitted MDR-TB genotypes.
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