A renewable enzyme-driven dissipative DNA strand displacement strategy for T4 polynucleotide kinase activity assay and inhibitor screening

, respectively. Furthermore, the inhibitory effects of two compounds were successfully evaluated using this method. Significantly, the reversibility of this dissipative system highlights its potential in consecutive analysis or dynamic monitoring. This strategy not only provides a promising approach for T4 PNK-related biological and medical research but also sheds new light on the application of dissipative systems in quantitative analysis.