猪链球菌
生物
遗传学
多位点序列分型
基因
血清型
系统发育树
基因型
基因组
打字
全基因组测序
微生物学
毒力
作者
Junkai Zhang,Yingying Yang,Huarun Sun,Xingwei Luo,Xiao-Die Cui,Qingqing Miao,Dongjian He,Jinfeng Zhao,Fengbin Yan,Yongchu Pan,Yajun Zhai,Gongzheng Hu
标识
DOI:10.1016/j.resmic.2023.104078
摘要
Aim of this study was to investigate the prevalence and genetic environment of the oxazolidinone resistance gene optrA in Streptococcus suis (S. suis) isolates from diseased pigs in China. A total of 178 S. suis isolates were screened for the optrA gene by PCR. The phenotypes and genotypes of optrA-positive isolates were investigated by antimicrobial susceptibility testing, core genome Multilocus Sequence Typing (cgMLST), capsular serotypes determination and whole-genome sequencing (WGS). Fifty-one (28.7%) S. suis isolates were positive for optrA. Phylogenetic analysis indicated that the spread of the optrA among S. suis isolates was primarily due to horizontal transfer. Analysis of S. suis serotypes from diseased pigs revealed substantial diversity. The genetic environment of optrA was complex and diverse and could be divided into 12 different types. Interestingly, we identified a novel integrative and conjugative element ICESsu988S, carrying optrA and erm(T) genes. This is to the best of our knowledge the first report of the optrA and erm(T) co-located on an ICE in S. suis. Our results showed a high prevalence of optrA gene in S. suis isolates in China. Further research is needed to evaluate the importance of ICEs, as they horizontally propagate important clinical resistance genes.
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