微泡
间充质干细胞
流式细胞术
外体
FOXP3型
细胞生物学
CD28
白细胞介素2受体
免疫印迹
CD63
生物
分子生物学
化学
CD8型
T细胞
免疫学
免疫系统
小RNA
生物化学
基因
作者
Liyan Guo,Peilong Lai,Suxia Geng,Xiaomei Chen,Yulian Wang,Tian Huang,Xin Du,Jianyu Weng
出处
期刊:PubMed
日期:2019-02-01
卷期号:27 (1): 221-226
被引量:2
标识
DOI:10.7534/j.issn.1009-2137.2019.01.036
摘要
To investigate the effects of exosomes from human umbilical cord mesenchymal stem cells on the development of Treg and TH17 cells.Exosomes from the serum-free-culture supernatants of hUC-MSC were harvested by ultracentrifugation. The electron microscopy, nanoparticle tracking analysis and western blot were used to identify the hUC-MSC-exosomes, such as the morphology, the paticle chameter, and the protein content. The PBMC stimulated with anti-CD3/CD28 were incubated with the exosomes for five days, and then the percentage changes of Treg and TH17 cells were analyzed by using flow cytometry.The hUC MSC-derived exosomes were saucer-like in morphology the averge diameter was approximately 142 nm. They were identified as positive for CD9 and CD63. Flow cytometry showed that the proportion of CD4+CD25+Foxp3+ Treg cells in the PBMC were significantly higher, but the proportion of CD4+IL17A+ T cells in the hUC-MSC-exosome group was obviously lower than that in the group without the hUC-MSC-exosom (control group) (P<0.05).The hUC-MSC-exosomes have an immunomodulatory effect on T cells in vitro by increasing the ratio of Treg and reducing the ratio of TH17 cells, expecting the hUC-MSC-exosom as a novel cell-free target for immunotherapy.
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