化学
电化学
氧化还原
儿茶酚
抗坏血酸
循环伏安法
检出限
玻璃碳
分子内力
石墨烯
支撑电解质
伏安法
无机化学
电极
立体化学
色谱法
物理化学
有机化学
化学工程
食品科学
工程类
作者
Dongdong Zhang,Jiatao Chen,Chen Xia,Xiuting Ding,Yanmin Zhang
标识
DOI:10.1016/j.jelechem.2023.117752
摘要
In this paper, the cyclic voltammetric behavior of dopamine (DA) was investigated systematically at different potential windows on electrochemical reduce graphene oxide modified glassy carbon electrode, and we found that the electrochemical redox mechanism of DA is a multi-step ECE process and the redox peak appearing at low potentials (∼–0.30 V) was dependent on the oxidation of DA at high potentials (∼0.20 V). The electrolyte enriched with the DA preoxidation product was then analyzed by LC-MS/MS and its structure was identified as dopaminochrome (DC). So, we proposed a new insight for the non-oxidative electrochemical analysis of DA by reverse voltammetry preoxidation and intramolecular cyclization. Compared with the traditional forward scaning mode, the approach could minimize the interference of ascorbic acid (AA) and enable the dual quantitative analysis of DA in the presence of high concentration of AA with the linear range of 2.0 to 80 μM and 60 to 200 μM, respectively, and a detection limit of 1.0 μM. Furthermore, the voltammetric properties of norepinephrine (NE), epinephrine (EP), 3,4-dihydroxy-l-phenylalanine (l-DOPA), 4-O-methyldopamine (HMP) were also investigated. The similar voltammetric properties to DA of NE, EP, l-DOPA and HMP suggested that the proposed reverse voltammetric preoxidation method is generalizable and equally applicable to monoamine neurotransmitters with a catechol structure.
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