双金属片
检出限
纳米材料
免疫分析
材料科学
纳米技术
线性范围
纳米颗粒
微电极
电化学气体传感器
生物传感器
化学
电化学
癌胚抗原
安培法
催化作用
辣根过氧化物酶
电极
色谱法
有机化学
酶
医学
抗体
癌症
物理化学
内科学
免疫学
生物
作者
Xia Cao,Ning Wang,Shiru Jia,Lin Guo,Ké Li
标识
DOI:10.1016/j.bios.2012.07.046
摘要
The recent development in the nanotechnology has paved the way for a large number of alloyed nanomaterials and devices of desirable properties which have useful functions for electrochemical sensor and biosensor applications. In this paper, bimetallic AuPt nanochains were synthesized through a mild chemical method, with which anti-horseradish peroxidase-conjugated anti-carcinoembryonic antigen (HRP-anti-CEA-NCAuPt) was developed for electrochemical detection of carcinoembryonic antigen (CEA) in a sandwich-type immunoassay format. The alloyed nanocrystals exhibit not only sound signal amplification effect of Au nanoparticles, but also further new combination of interfacial, electrical and structural properties arising from the disparate AuPt components. As a result, the electrochemical signal was significantly amplified by using the HRP-anti-CEA-NCAuPt as tracer and hydrogen peroxide as enzyme substrate. The linear range of the developed immunosensor is 0.01–200 ng/mL and the detection limit is 0.11 pg/mL of CEA, which makes the biometallic nanochains promising candidates for the next-generation sandwich-type electrochemical immunoassays.
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