检出限
癌胚抗原
肉眼
碳纳米管
线性范围
材料科学
化学
纳米技术
核化学
化学工程
色谱法
医学
癌症
内科学
工程类
作者
Weiyan Liu,Hongmei Yang,Yanan Ding,Shenguang Ge,Jinghua Yu,Mei Yan,Xianrang Song
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2014-01-01
卷期号:139 (1): 251-258
被引量:62
摘要
A new paper-based colorimetric immunosensor for the detection of carcinoembryonic antigen (CEA) was developed based on the intrinsic peroxidase activity of ZnFe2O4-multiwalled carbon nanotubes (ZnFe2O4@MWNTs). The immunosensor platform was prepared by depositing chitosan and porous gold onto filter paper and entrapping the primary antibodies (Ab1) onto the layers. Secondary antibodies (Ab2) were assembled on the surface of the functionalized ZnFe2O4@MWNTs. The immunosensor response was quantified as a color change resulting from ZnFe2O4@MWNTs catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of H2O2. The catalytic performance of ZnFe2O4@MWNTs was higher than ZnFe2O4 due to the high electrical conductance of MWNTs, moreover, the electron communications between ZnFe2O4@MWNTs and substrates are electrically "wired". Detection was achieved by measuring the color change when the concentrations of CEA were different. The color change can be quantified with the naked eye but a digitalized picture can also be used to provide more sensitive comparison to a calibrated color scheme. This method was simple for CEA detection with a linear range from 0.005 to 30 ng mL(-1) and a detection limit of 2.6 pg mL(-1). Such an equipment-free immunoassay has great potential in resource-limited environments.
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