次氯酸钠
生理盐水
化学
洗必泰
生物相容性
体内
西替利
牙科
医学
麻醉
生物
生物技术
有机化学
作者
Luciana Stadler Demenech,Flávia Sens Fagundes Tomazinho,Flares Baratto‐Filho,João Armando Brancher,Luiz Fernando Pereira,Marilisa Carneiro Leão Gabardo
摘要
Abstract The biocompatibility of irrigant solutions, including 8.25% sodium hypochlorite (NaOCl), was analyzed in samples ( n = 25) of chorioallantoic membranes (CAM) from embryos of the Gallus gallus , using the hen's egg test on chorioallantoic membrane (HET‐CAM). The following irrigants were tested: saline (control), 2% chlorhexidine (2% CHX), and NaOCl (2.5%, 5.25%, and 8.25%), and morphological and microcirculatory changes were investigated. Images were selected and quantified with Image J®. The macroscopic data were submitted to ANOVA, followed by the Tukey's test ( p < .05); non‐parametric Kruskal‐Wallis and Mann–Whitney tests were applied to histological data ( p < .05). The frequency of hyperemia differed significantly in the 8.25% NaOCl compared to the other groups, with the exception of 5.25% NaOCl ( p = .096). Coagulation was more frequent in the 2% CHX group, differing significantly from the 2.5% NaOCl group ( p = .038). In mean values, NaOCl 8.25% caused the decrease (NaOCl = 2.60, and control = 5.00) of vessels in the microcirculation, as well as triggered the increase (NaOCl = 425.50, and control 44.50) of inflammatory cells in the observed areas. There was necrosis in all samples in the 5.25% NaOCl group and in 80% in the 2% CHX, 2.5% NaOCl, and 8.25% NaOCl groups. Fibroblasts were detected only in the control group. It was concluded that the 8.25% NaOCl solution showed significantly high toxicity on CAM. However, these results were comparable to the other concentrations of NaOCl and to 2% CHX. Thus, in this experimental protocol, 8.25% NaOCl proved to be viable as an irrigant solution.
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