喹啉
化学
荧光团
检出限
溶酶体
荧光
组合化学
选择性
三唑
硫化氢
猝灭(荧光)
分析物
点击化学
生物化学
酶
色谱法
有机化学
催化作用
物理
硫黄
量子力学
作者
Yandong Dou,Xingyu Gu,Shasha Ying,Shuhua Zhu,Shian Yu,Wei Shen,Qing Zhu
摘要
A fluorogenic probe based on the novel fluorophore 5-triazole-quinoline was developed for the detection of hydrogen sulfide, an endogenous signaling molecule associated with the development of various diseases. The lysosome-targeted probe Lyso-HS was synthesized via C-H direct azidation from 8-aminoquinoline; it was able to detect H2S in 1 min and exhibited excellent turn-on ability with 95-fold fluorescence enhancement based on a new fluorochrome. The high quenching efficiency was further verified using time-dependent density functional theory (TDDFT). The probe also exhibited high selectivity and a low detection limit (as low as 214.5 nM), which has practical applications for disease detection and monitoring.
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